Uncovering the role of immunosuppressive dendritic cells in pancreatic cancer

Uncovering the role of immunosuppressive dendritic cells in pancreatic cancer

Dwayne L. Thomas II1,2,3, Adrian G. Murphy1

1Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA

Correspondence to: Adrian G. Murphy. Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Email: amurph39@jhmi.edu.

Comment on: Kenkel JA, Tseng WW, Davidson MG, et al. An Immunosuppressive Dendritic Cell Subset Accumulates at Secondary Sites and Promotes Metastasis in Pancreatic Cancer. Cancer Res 2017;77:4158-70.

Received: 31 July 2018; Accepted: 14 September 2018; Published: 30 September 2018.

doi: 10.21037/apc.2018.09.02

Pancreatic ductal adenocarcinoma (PDAC) is a debilitating disease that continues to have rising incidence and mortality rates. In 2018, it is estimated that nearly 56,000 individuals will be newly diagnosed with pancreatic cancer (1). Surgical resection remains the only possible curative measure as pancreatic cancer is notoriously resistant to systemic chemotherapy, has high rates of recurrence, and is associated with a 5-year survival rate of 8.5 (1). Increasingly, current research focuses on the immune system’s interactions within the tumor microenvironment and exploring different ways to prime that environment to stimulate an immune response. Novel immunotherapies have the potential to harness the patient’s innate immune system to help combat pancreatic cancer (2,3). This has led to numerous exciting discoveries and the development of novel therapies targeting different immune checkpoint pathways and the microenvironment.

The inherent complexity of the immune system results in certain immune components protecting against cancer (tumor infiltrating lymphocytes) (4) where as others function by promoting immune suppression such as myeloid-derived suppressor cells (MDSC) and tumor-derived macrophages (TAM) (5,6). The relatively poor response rates to immunotherapy in pancreatic cancer compared to lung cancer and melanoma, have prompted efforts to further elucidate the specific components of the immune system’s response in the tumor microenvironment including the immune response at sites of metastasis.

Kenkel et al. investigated how the immune response at secondary/metastatic sites affected the metastatic behavior of pancreatic cancer in a preclinical model of PDAC (7). Using an immune competent orthotopic mouse model and Pdx1-Cre; Kras; Trp53 mutated murine pancreatic cancer cells, CD11b+ myeloid cells were found to have accumulated in the liver, surrounding early metastatic sites. Upon further characterization, these CD11b+ (CD11b+ CD11chi MHC-IIhi) cells were categorized as dendritic cells (DCs), which secreted high levels of pro-tumoral mediators such as IL6, TNFα, and CCL2. DCs in tumor-bearing mice showed higher levels of expression of the immune checkpoint molecules PD-L1, PD-L2, and ICOSL when compared to normal liver DCs. The CD11b+ DCs expressed multiple immunosuppressive factors resulting in the protection of metastases from immune elimination. This specific population of CD11b+ DCs expressed monocyte/macrophage marker CD115, suggesting that they were derived from monocytes. Kenkel et al. further tested this theory by performing adoptive transfer of bone marrow monocytes that were congenically marked (CD45.1) into tumor-bearing mice. Before the transfer, these monocytes had high expression levels of Gr1/Ly6C, but five days post transfer the cells no longer expressed Gr1. This finding indicated that these monocytes were observed to traffic directly to the liver and had differentiated into CD11b+ DC. As CD11b+ DC accumulated in the liver, they were shown to increase regulatory T-cells in comparison to the poor response of CD8+ T-cells. Additionally, CD11b+ DCs uniquely expressed PD-L2 and anti-PD-L2 blocking antibodies lead to reduced metastatic progression via regulatory T cells expansion, which was mediated, by CD8+ T cells (7). This research suggested a potential pathway for future targeting in an effort to enhance CD8+ T cell activity resulting in growth at secondary/metastatic sites.

DCs are a unique subset of antigen presenting cells that capture, internalize, and process antigens in vivo (8-12) allowing for presentation on major histocompatibility complex (MHC) molecules and recognition by T cells (9-12). To date, their role in PDAC has been limited to their involvement in passive immunotherapy approaches (13).

This study demonstrates the potential for targeting key components in the innate immune system which can have favorable effects on tumor growth and metastasis. It also highlighted the specific influence of GM-CSF producing tumor cells which facilitated DC accumulation at metastatic sites. GM-CSF is increasingly known for its role in DC maturation (14) and directly upregulates major signaling pathways including JAK/STAT, MAPK and PI3K. Given the frequent use of recombinant G-CSF in patients with PDAC, the role of exogenous G-CSF on this specific DC subset is of particular interest (15).

This paper highlights the significance of how the immunosuppressive CD11b+ DCs play a critical role in the early metastasis of pancreatic cancer at metastatic/secondary sites. In particular, these cells expressed MGL2 and PD-L2 and inhibiting these molecules resulted in the depletion of this DC subset thereby enhancing tumor immunity and inhibiting metastasis. MGL (macrophage galactose type C-type lectin) is expressed in human DCs and macrophages and has been shown to bind to epithelial mucins (MUC1) in colon cancer. Further study is required to elucidate the role of MGL (16,17) and its interactions with MUC1 in pancreatic cancer. This study also confirmed that this specific DC subset does exist in human PDAC where CD11c+HLA-DR+ cells were observed to accumulate near PD-L2+ stromal cells in cases of human PDAC liver metastases. These stromal cells were also observed to express MGL (the human homolog of MGL2). To date, there has been more focus on PD-1/PD-L1 interactions with anti-PD1 therapies, although there is emerging evidence that PD-L2 expression may play a role in disease response suggesting that therapies, which target both PD-L1 and PD-L2 may be more beneficial (18).

While the molecular targets of CD11b+ DCs have been established in this preclinical model, the challenge remains to translate these findings into therapeutic strategies targeting immunosuppressive DCs for patients with PDAC. This study suggests that the differential immune responses at metastatic sites vs. primary sites merits further attention, particularly to explain the underlying mechanism of immune suppression in PDAC.


Funding: None.


Provenance and Peer Review: This article was commissioned by the editorial office, Annals of Pancreatic Cancer. The article did not undergo external peer review.

Conflicts of Interest: Both authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/apc.2018.09.02). The authors have no conflicts of interest to declare.

Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.


  1. Institute NC. Cancer Stat Facts: Pancreatic Cancer 2018. 2018. Available online: https://seer.cancer.gov/statfacts/html/pancreas.html
  2. Jaffee EM, Hruban RH, Biedrzycki B, et al. Novel allogeneic granulocyte-macrophage colony-stimulating factor-secreting tumor vaccine for pancreatic cancer: a phase I trial of safety and immune activation. J Clin Oncol 2001;19:145-56. [Crossref] [PubMed]
  3. Lutz E, Yeo CJ, Lillemoe KD, et al. A lethally irradiated allogeneic granulocyte-macrophage colony stimulating factor-secreting tumor vaccine for pancreatic adenocarcinoma. A Phase II trial of safety, efficacy, and immune activation. Ann Surg 2011;253:328-35. [Crossref] [PubMed]
  4. Ino Y, Yamazaki-Itoh R, Shimada K, et al. Immune cell infiltration as an indicator of the immune microenvironment of pancreatic cancer. Br J Cancer 2013;108:914-23. [Crossref] [PubMed]
  5. Mantovani A, Schioppa T, Porta C, et al. Role of tumor-associated macrophages in tumor progression and invasion. Cancer Metastasis Rev 2006;25:315-22. [Crossref] [PubMed]
  6. Kusmartsev S, Gabrilovich DI. Role of immature myeloid cells in mechanisms of immune evasion in cancer. Cancer Immunol Immunother 2006;55:237-45. [Crossref] [PubMed]
  7. Kenkel JA, Tseng WW, Davidson MG, et al. An Immunosuppressive Dendritic Cell Subset Accumulates at Secondary Sites and Promotes Metastasis in Pancreatic Cancer. Cancer Res 2017;77:4158-70. [Crossref] [PubMed]
  8. Steinman RM, Banchereau J. Taking dendritic cells into medicine. Nature 2007;449:419-26. [Crossref] [PubMed]
  9. Platt CD, Ma JK, Chalouni C, et al. Mature dendritic cells use endocytic receptors to capture and present antigens. Proc Natl Acad Sci U S A 2010;107:4287-92. [Crossref] [PubMed]
  10. Chen J, Namiki S, Toma-Hirano M, et al. The role of CD11b in phagocytosis and dendritic cell development. Immunol Lett 2008;120:42-8. [Crossref] [PubMed]
  11. Macri C, Dumont C, Johnston AP, et al. Targeting dendritic cells: a promising strategy to improve vaccine effectiveness. Clin Transl Immunology 2016;5:e66 [Crossref] [PubMed]
  12. Trombetta ES, Mellman I. Cell biology of antigen processing in vitro and in vivo. Annu Rev Immunol 2005;23:975-1028. [Crossref] [PubMed]
  13. Mehrotra S, Britten CD, Chin S, et al. Vaccination with poly(IC:LC) and peptide-pulsed autologous dendritic cells in patients with pancreatic cancer. J Hematol Oncol 2017;10:82. [Crossref] [PubMed]
  14. van de Laar L, Coffer PJ, Woltman AM. Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy. Blood 2012;119:3383-93. [Crossref] [PubMed]
  15. Rossetti M, Gregori S, Roncarolo MG. Granulocyte-colony stimulating factor drives the in vitro differentiation of human dendritic cells that induce anergy in naïve T cells. Eur J Immunol 2010;40:3097-106. [Crossref] [PubMed]
  16. Hinoda Y, Ikematsu Y, Horinochi M, et al. Increased expression of MUC1 in advanced pancreatic cancer. J Gastroenterol 2003;38:1162-6. [Crossref] [PubMed]
  17. Saeland E, van Vliet SJ, Bäckström M, et al. The C-type lectin MGL expressed by dendritic cells detects glycan changes on MUC1 in colon carcinoma. Cancer Immunol Immunother 2007;56:1225-36. [Crossref] [PubMed]
  18. Yearley JH, Gibson C, Yu N, et al. PD-L2 Expression in Human Tumors: Relevance to Anti-PD-1 Therapy in Cancer. Clin Cancer Res 2017;23:3158-67. [Crossref] [PubMed]
doi: 10.21037/apc.2018.09.02
Cite this article as: Thomas DL 2nd, Murphy AG. Uncovering the role of immunosuppressive dendritic cells in pancreatic cancer. Ann Pancreat Cancer 2018;1:28.

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